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. 1997 Apr 7;137(1):247–258. doi: 10.1083/jcb.137.1.247

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Effect of protein tyrosine kinase inhibitor on c-src/tubulin association. Avian marrow macrophages maintained in suspension for 2 d were exposed for 2 h to the tyrosine kinase inhibitor (herbimycin-A 4 μM). Cells, in the presence of the drug, were kept suspended or plated on tissue culture plastic for 90 min. They were then lysed and immunoprecipitated with anti–c-src or -tubulin antibodies. Proteins were separated by SDSPAGE, transferred to nitrocellulose membranes, and probed with the anti–c-src antibody.