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. 1997 Apr 7;137(1):247–258. doi: 10.1083/jcb.137.1.247

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Tubulin-bound and nonbound c-src in adherent cells. The lysate of adherent avian marrow macrophages was immunoprecipitated with antitubulin antibody (lane 1), and the supernatant was reimmunoprecipitated with the same antibody (lane 2). The supernatant, now depleted of tubulin-associated c-src, was immunoprecipitated with anti–c-src antibody (lane 3). All lanes were probed with anti–c-src antibody.