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. 1997 Apr 7;137(1):67–77. doi: 10.1083/jcb.137.1.67

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Pro-GelA and proGelB activation by MT1MMP and ST1, in the absence or presence of TIMPs. (A) COS-1 cells were transiently cotransfected with expression plasmids for MMPs whose transcripts were found to be expressed during rat skin wound healing (Figs. 1 and 3), according to the following combinations: pTLlGelA and pTL1 (lane 1) or pTL1MT1MMP (lane 2) or pTL1ST1 (lane 3) or pTL1ST3 (lane 4) or pTL1GelB (lane 5); pTL1GelB and pTL1MT1MMP (lane 6) or pTL1ST1 (lane 7) or pTL1ST3 (lane 8) or pTL1 (lane 9). (B) COS-1 cells were transiently cotransfected with pTL1GelA and pTL1 (lane 1), or pTL1GelA and pTL1MT1-MMP (lanes 2–5), or pTL1GelB and pTL1 (lane 6), or pTL1GelB and pTL1ST1 (lanes 7–10), in the absence (lanes 1, 2, 6, and 7) or presence of one of the TIMP expression plasmids: pTL1TIMP1 (lanes 3 and 8), pTL1TIMP2 (lanes 4 and 9), pTL1TIMP3 (lanes 5 and 10). In A and B, 20 μl of each conditioned medium was analyzed by gelatin zymography.