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. 1997 Apr 7;137(1):67–77. doi: 10.1083/jcb.137.1.67

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Gelatinolytic activities during rat skin wound healing. Tissues were microdissected from cryostat sections, incubated with SDS sample buffer, and subjected to gelatin zymography. (A) Normal skin containing both epidermis and dermis (lane 1), and healing skin wounds at days 1, 3, 5, 7, 10, and 14 after cutaneous incision (lanes 2–7). (B) Normal skin adjacent to the wound tissue and containing both epidermis and dermis (N) (lane 1), and granulation tissue (G) (lanes 2 and 4) or proliferative epithelial layer (E) (lanes 3 and 5) of healing skin wounds at days 3 (lanes 2 and 3) and 5 (lanes 4 and 5) after cutaneous incision. In A and B, proteins extracted from ∼1 mg of fresh tissue were analyzed in each lane.