Table I.
Comparison of Various β1-Integrin Antibody Treatments on Colony Size in 3-Dimensional Culture
| Description | Mean colony size | Relative size ratio of T4-2/S-1 colonies* | ||
|---|---|---|---|---|
| μm | ||||
| S-1 50 cells | 24.5 ± 0.7 | N/A | ||
| Control T4-2 cells (nonspecific rat IgG's)‡ | 74.3 ± 2.4 | 3.03 ± 0.03 | ||
| Inhibitory β1-antibody-treated cells, clone AIIB2 crude ascites | 24.1 ± 1.0 | 0.98 ± 0.10 | ||
| Inhibitory β1-antibody-treated cells, clone AIIB2 purified IgG1 | 28.2 ± 1.1 | 1.15 ± 0.04 | ||
| Inhibitory β1-antibody-treated cells, clone AIIB2 Fab fragments | 24.5 ± 1.4 | 1.04 ± 0.04 | ||
| Inhibitory β1-antibody-treated cells, clone JB1a crude ascites (alternate inhibitory mAb) | 32.3 ± 1.4 | 1.32 ± 0.06 | ||
| Activating β1-antibody-treated cells, clone TS2/16 crude ascites (stimulatory mAb) | 61.7 ± 3.3 | 2.52 ± 0.13 |
*
The diameter of 200 S-1 acini as observed directly by phase contrast microscopy, were measured after 12 d in 3-D culture using an eyepiece equipped with a micrometer spindle. The same measurements were made on 100–400 T4-2 colonies in the presence of 100 μg antibody ascites protein or 25 μg purified IgG/ml EHS matrix of various β1-integrin antibodies (listed above). The average measurements of each of these groups ± SEM were calculated and the ratio of T4-2/S-1 diameters ± SEM were determined.
‡
Comparable results were also obtained with nonfunction altering antibodies α6- (clone- J1B5) and β1-integrin (clone DF5), which are not shown.