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. 1997 May 5;137(3):619–631. doi: 10.1083/jcb.137.3.619

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Importance of the spacing of Glu residues. (A) A series of clones, based on the mutant CONS.C^2xE, were constructed varying the spacing between two Glu residues, from a minimum spacing of two residues (diad mutant) to a maximum spacing of eight residues (octad mutant). (B) Transformation by each isolate shown in (A) was quantitated as a percentage of p185^neu. Numerical results of transformation assays are presented graphically and represent the average values from two independent experiments, normalized by cotransfection with pSV2neo.

Importance of the spacing of Glu residues. (A) A series of clones, based on the mutant CONS.C^2xE, were constructed varying the spacing between two Glu residues, from a minimum spacing of two residues (diad mutant) to a maximum spacing of eight residues (octad mutant). (B) Transformation by each isolate shown in (A) was quantitated as a percentage of p185^neu. Numerical results of transformation assays are presented graphically and represent the average values from two independent experiments, normalized by cotransfection with pSV2neo.