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. 1997 May 5;137(3):595–608. doi: 10.1083/jcb.137.3.595

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Inefficient arrival of newly synthesized ProL in lysosomes of INS cells. Cells pulse labeled as in Fig. 1 were chased to 3 h before a further 3-h collection of stimulated (+) or unstimulated (−) secretion was obtained and the cells analyzed by immunoprecipitation for lysosomal arrival of cathepsin L. Stimulus-dependent secretion contained ∼50% of all immunoprecipitable L-containing peptides found in the 3 h of collected medium plus cell lysate, while insulin secretion as measured by RIA averaged 53% during this period. The positions of ProL and bands comprising mature cathepsin L (large bracket) are shown.