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. 1997 May 5;137(3):657–669. doi: 10.1083/jcb.137.3.657

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Immunoblot analysis with affinity-purified antiBSN UC56, 372, and 176 antibodies. (A) Total proteins were isolated from mouse testis and skin, and samples were resolved by electrophoresis through 8.5% SDS– polyacrylamide gels. Proteins were transferred by electroblotting to Immobilon-P membranes (Millipore Corp., Bedford, MA), and blots were subjected to immunoblot analysis as described by the manufacturer. Each of the three anti-BSN peptide antibodies were affinity column purified before use. In both testis and skin samples, a single band of 120 kD was detected; this band was not detected by secondary antibody alone or by preimmune sera. (B) Total proteins were isolated from a variety of adult mouse tissues and subjected to immunoblot analysis as outlined in A. Abbreviations are as indicated in the legend to Fig. 3, except: hEP, human epidermal keratinocytes; BR, brain.