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. 1997 May 5;137(3):657–669. doi: 10.1083/jcb.137.3.657

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Fractionation of sperm proteins confirms presence of the majority of basonuclin in the acrosome and sperm tail. Sperm were isolated from the epididymis of adult mice. They were first stained with αBSN UC56 antibodies to confirm the presence of acrosomal (ac) and middle piece (mp) staining in mature sperm (two sperm shown in A; bottom middle piece has a 90° kink). Sperm were then fractionated by sonication and sucrose gradient centrifugation as described by Walensky and Snyder (1995). Proteins were solubilized in 10 mM DTT, 2% SDS, and samples were resolved by electrophoresis through 8.5% SDS–polyacrylamide gels. Gels were analyzed by either staining with Coomassie blue (B) to visualize total proteins or immunoblot analysis (C) with antibodies against basonuclin or PLCβ1. E, total protein extract from epidermal keratinocytes; S, total sperm proteins; A, acrosomal fraction; T, tail fraction; N, nuclear fraction. Note: Sperm centrioles were lost in the fractionation procedure, as judged by immunofluorescent staining of each fraction.