Figure 6.

CCCP attenuates the Ca²⁺ plateau and inhibits loading of the TG-insensitive store. (A) 2 mM Ca²⁺ was readded to TGtreated cells in the presence (dotted trace) or absence (solid trace) of 1 μM CCCP. After termination of the store loading period, ionomycin was added to assay store content. (B) Average response of CCCP-treated cells selected for a final [Ca²⁺]_i plateau >1 μM from the experiment shown in A (43/264 cells). CCCP prevents store loading even if [Ca²⁺]_i is high. (C) 1 μM CCCP was applied at different times during the plateau response in 2 mM Ca²⁺. Each trace represents the average of 250–300 cells.