Table I.
Effects of Mitochondrial Inhibitors on the Ca2+ Plateau and Loading of the TG-insensitive Store
| Plateau [Ca2+]i | Store content | |||||||
|---|---|---|---|---|---|---|---|---|
| Jurkat | Human T cells | Jurkat | Human T cells | |||||
| nM | nM | |||||||
| Control (2 mM Ca2+ o) | 1,003 ± 249 (14) | 1,100 (1) | 1,412 ± 555 (14) | 3,052 (1) | ||||
| + CCCP | 399 ± 110 (6) | 623 (1) | 116 ± 15 (3) | 76 (1) | ||||
| + Antimycin A1 | 395 ± 137 (6) | 400 (1) | 626 ± 43 (3) | 1,033 (1) | ||||
| + Antimycin A1/oligomycin | 298 (1) | ND | 60 (1) | ND | ||||
| − Na | 334 ± 64 (5) | 279 (1) | 1,731 ± 699 (2) | 1,929 (1) | ||||
Plateau [Ca2+]i was measured 1,000 s after readdition of Ca2+ to TG-treated cells. The store content of the TG-insensitive store is expressed as the peak [Ca2+]i after addition of ionomycin (see Fig. 2). All values are given as a mean ± SD (number of experiments), and 250–300 cells were analyzed for each experiment.