Table I.

Effect of Phosphorylation on Pro-uPA–promoted Adherence of TGF-β/Vitamin D₃ Primed THP-1 and HL-60 Cell Lines

Effector		THP-1				HL-60
		Adherent cells*		SD‡		Adherent cells		SD
		%				%
None		4.55		0.73		6.5		0.43
His-pro-uPAwt		24.17		5.07		21.17		1.2
His-pro-uPA138E		9.77		2.03		10.02		0.55
His-pro-uPA138E/303E		5.2		1.65		5.7		1.4
DFP-His-pro-uPAwt		nd		−		22.1		1.23
DFP-His-pro-uPA138E		nd		−		10.87		0.34
DFP-His-pro-uPA138E/303E		nd		−		6.35		0.88
DFP-Pser-uPA		9.92		2.4		9.02		1.0
DFP-Ser-uPA		17.75		1.96		18.52		1.4

^* THP-1 and HL-60 cells were treated with 1 ng/ml TGF-β and 50 nM dihydroxyvitamin D₃ for 20 h, and subsequently incubated with 0.2 nM of the indicated effectors for 30 min. Numbers refer to percentages of adherent cells relative to total cell number. The data represent the average of three experiments performed in duplicate.  

^‡ SD, standard deviation.