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ypt31-Δ/ypt32-A141D mutant cells do not exhibit a major defect in targeting of CPY to the vacuole at the nonpermissive temperature. Extracts from the 37°C samples shown in Fig. 4 were subjected to precipitation with anti-CPY antibodies and resolved on 8% SDS–polyacrylamide gels. Positions of the ER (p1), Golgi (p2), and vacuolar (m) forms of CPY are indicated in the left margin.
