Figure 1.

Agrobacterium tumefaciens binary vector used for coexpression of catalytic (CNA) and regulatory (CNB) subunits for reconstitution of activated yeast calcineurin (CaN) in transgenic tobacco. The gene encoding a truncated form of the yeast CNA2 subunit deleted after Thr-459 (CNAtr) was inserted into the SalI site of pBTEX. The marker gene in this vector encodes for neomycin phosphotransferase (npt-II) for kanamycin selection driven by the nopaline synthase gene promoter and terminator (not shown). The CNB1 cDNA was inserted between the cauliflower mosaic virus 35S promoter and Ocs terminator in plasmid pTEX by using the XbaI and SalI sites. Subsequently, the entire cassette was cloned into the HindIII site of pBTEX containing CNAtr to obtain the binary plasmid pBTCaN. Cloning sites are indicated as follows: HindIII (H), SalI (S), and XbaI (X).