Figure 2.

Expression of yeast CNAtr and CNB1 transcripts in transgenic tobacco plants. Total RNA was isolated from leaves of seed-derived untransformed W38 plants (W) and T₀ plants regenerated after transformation with plasmid pBTCaN and kanamycin selection (A2, A9, C8, T7, and F3) and from cells of yeast cna1 cna2 double-disputant MCY100 transformed with plasmid pCAtrB, also containing CNAtr and CNB1 (Y) (2). Ten micrograms of RNA was loaded into each lane, transferred to nitrocellulose filters, and hybridized with CNAtr (Upper) or CNB1 (Lower) insert. Approximate transcript sizes of CNAtr and CNB1 are 1.4 and 0.5 kb, respectively.