Figure 3.

Calcineurin expression based on immunodetection of CNB. Illustrated is the immunoblot of 15 μg protein extracted from the Δcnb1 yeast disruptant YP9 (Δcnb1), wild-type yeast DBY746 (w.t.), or DBY746 transformed with plasmid pCAtrB for overproduction of CNB (pCAtrB), 0.5 μg purified recombinant CNB protein (CNBp), and 45 μg protein isolated from leaves of T₂ plants homozygous for npt-II (A9_hom, T7_hom), the complementary azygous (A9_azy, T7_azy), or homozygous T₂ plants transformed with an empty plasmid (vector). Proteins were separated on a 15% SDS-polyacrylamide gel and reacted with chicken anti-CNB antibodies.