Figure 4.
Activated yeast CaN mediates NaCl tolerance of tobacco plants. Homozygous T2 plants from lines PKY (expressing only npt-II) and A9 (expressing CNAtr and CNB1 as well as npt-II) were treated with 200 mM NaCl. After 7 days of salt shock, plants were transferred to fresh nutrient solution without NaCl and left to recover for 12 days. (Upper) Percentage of surviving seedlings of 36 progeny from each line (significantly different at the 95% level) and growth parameters of all plants surviving the NaCl treatment ± SE (6 and 28 plants from lines PKY and A9, respectively). Average fresh weights of PKY and A9 plants before the NaCl treatment were 1.63 ± 0.11 g and 1.76 ± 0.10 g, respectively. (Lower) Three representative plants from lines A9 (top row) and PKY (bottom row) that survived NaCl shock and after the recovery period.