Figure 10.

Relative composition profiles of molecular species of myocardial GPA, GPGro, and FFA classes in control and diabetic mice. Myocardial lipid extracts from control and diabetic mice were prepared by a modified Bligh and Dyer procedure as described under Materials and Methods. The quantities of individual molecular species of GPGro (panel A), GPA (panel B), and FFA (panel C) in myocardial lipid extracts of control which is averaged from both control of STZ-treated mice (4−6 months of age) and WT mice at 4 months of age (open bars), mice for 40 days after STZ treatment (solid bars), and ob/ob mice at 4 months of age were determined by shotgun lipidomics by comparison of the peak intensity of each individual ion to that of the selected internal standards after corrections for ¹³C isotopologue distribution differences as described under Materials and Methods. The displayed relative composition of individual molecular species to total content of the related class represents the mean ± SEM from at least four different animals, and only those that are present in more than 5 mol % relative abundance of each class are displayed.