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. Author manuscript; available in PMC: 2007 Dec 14.
Published in final edited form as: Biochemistry. 2007 May 8;46(21):6417–6428. doi: 10.1021/bi7004015

Figure 4.

Figure 4

Dramatic changes of cardiolipin molecular species containing docosahexaenoate (22:6 FA) in STZ-induced diabetic mouse myocardium relative to controls. Lipid extracts were prepared by using a modified Bligh and Dyer method as described under Materials and Methods. CL molecular species containing 22:6 FA are profiled by neutral loss of 155.1 units (corresponding to the loss of a 22:6 ketene from doubly charged CL molecular species). For negative-ion tandem mass spectrometry in the neutral loss mode by using a QqQ mass spectrometer, both first and third quadrupoles were coordinately scanned with a mass difference (i.e., neutral loss) of 155.1 units from doubly charged CL molecular species while the second quadruple was used as a collision cell. The spectra are normalized to the CL ion peak at m/z 747.5 which was not significantly changed as determined by primary ions (Figure 1). The spectra indicate the dramatic increase of CL molecular species containing 22:6 FA in diabetic mouse myocardium.