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. Author manuscript; available in PMC: 2007 Dec 14.
Published in final edited form as: Biochemistry. 2007 May 8;46(21):6417–6428. doi: 10.1021/bi7004015

Figure 5.

Figure 5

Expanded negative-ion electrospray ionization mass spectra of myocardial lipid extracts from ob/ob and control mice. Myocardial lipid extracts of wild-type (panel A) and ob/ob (4 months of age, panel B) mice were prepared by a modified Bligh and Dyer procedure as described under Materials and Methods. Negative-ion ESI mass spectra were acquired by using a QqTOF mass spectrometer as described under Materials and Methods. Both spectra displayed have been normalized to the CL internal standard (which is not included in the spectra). The asterisks indicate the majority of the doubly charged CL plus-one isotopologues whose ion peak intensities are utilized to quantify individual CL molecular species as described under Materials and Methods. Other unlabeled ion peaks correspond to deprotonated molecular species of other anionic phospholipids and ethanolamine glycerophospholipids.