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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Virology. 2007 May 11;366(1):107–116. doi: 10.1016/j.virol.2007.03.052

Figure 3. Resistance of transgenic plant lines that produce mutant CPs to systemic infection by TMV.

Figure 3

Protection assay for TMV infection in transgenic N. tabacum Xanthi-nn plants expressing wt CP (line 3646) or CP carrying mutations at amino acids Asp77 or Glu50. The plants were inoculated with the TMV-U1 virus (0.1 mg/ml), and disease development was scored as the percentage of plants that exhibit symptoms on upper leaves and accumulation of TMV in the systemic leaves was measured by ELISA using anti-CP antibody. ELISA was performed on samples from 10 plants for each line, as described (Bendahmane et al., 1997).