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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Virology. 2007 May 11;366(1):107–116. doi: 10.1016/j.virol.2007.03.052

Figure 5. Effect of mutant CPs expressed in transgenic BY2 cells on MP:GFP following TMV-MP:GFP infection.

Figure 5

(A) Patterns of accumulation of MP:GFP in BY-2 protoplasts inoculated with TMV-MP:GFP as described in Heinlein et al. (1995). Protoplasts were inoculated with TMV-MP:GFP RNA and the accumulation of MP:GFP was analyzed by fluorescence microscopy. Pictures were taken at 12, 15, and 24 hpi corresponding to early, mid and late stages of infection by TMV, respectively. MP:GFP patterns found at each stage are presented. (B) Quantification of the presence of each of the six paterns (above) of MP:GFP accumulation during TMV-MP:GFP infection in protoplast expressing mutant CPs at 10, 16, 24 and 36 hpi. The presence of each pattern was normalized for the total number of cells counted per point and expressed as a percent. The three groups of mutants CP with respect to their effect on MP accumulation are presented: group (1) CP mutants with postivie effects on MP accumulation similar to w.t. CP; group (2) CP mutants that lost the positive effect of w.t. CP on MP accumulation patterns; group (3) CP mutants that acquired negative effects on accumulation of MP.