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. 1997 Jul 14;138(1):119–130. doi: 10.1083/jcb.138.1.119

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Expression of Clb2 in cells arrested in interphase leads to phosphorylation of Gin4. A bar1 strain carrying an integrated copy of Clb2^Δ176 under the control of the gal1 promoter was grown in raffinose and arrested in interphase by incubation in the presence of α-factor for 3 h. The culture was then divided in half, and Clb2^Δ176 expression was induced in one half by transferring the cells to media containing galactose and α-factor. At the times indicated, samples were taken from each culture and used for an anti-Gin4 Western blot.