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. 1997 Jul 14;138(1):119–130. doi: 10.1083/jcb.138.1.119

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Gin4 is not required for the activation of cyclin-dependent kinase activity by Clb2. (A) A time course showing the appearance of Clb2-associated kinase activity during mitosis. Wild-type and Δgin4 cells were released from an α-factor arrest and then assayed for Clb2-associated kinase activity during the cell cycle as previously described (Kellogg and Murray, 1995). (B) The same samples used for the kinase assay in A were probed for Clb2 levels by Western blotting as previously described (Kellogg and Murray, 1995).