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. 1997 Jul 14;138(1):119–130. doi: 10.1083/jcb.138.1.119

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Gin4 from mitotic cells phosphorylates histone H1 and undergoes autophosphorylation in vitro. (A) Cells from the indicated strains were arrested in G1 with α-factor or in mitosis with benomyl. Extracts were then made from the arrested cells and Gin4 was immunoprecipitated and assayed for kinase activity using histone H1 as a substrate. (B) A control showing that the kinase activity present in Gin4 immunoprecipitates is not due to Cdc28. Wild-type or cdc28-4 cells were grown at 22°C and arrested in mitosis with benomyl. Gin4 was then immunoprecipitated from the arrested cells and kinase activity was measured either at 22°C or 37°C.