Table I.
Plasmid | Protein expressed | Insertion points or deletion, aa | ND10 elimination | |||
---|---|---|---|---|---|---|
pSVH | IE72+86 | wt | + | |||
pSVCC3 | IE72 | wt | + | |||
pIE86 | IE86 | wt | − | |||
pIE55 | IE55 | wt | − | |||
Insertion mutants | ||||||
pSVH m1 | IE72+86 | 32 | + | |||
pSVH m2 | IE72+86 | 59 | + | |||
pSVH m16 | IE72+86 | 325 | + | |||
pSVH m17 | IE72+86 | 325 | + | |||
pSVH m18 | IE72+86 | 358 | + | |||
pSVH m19 | IE72+86 | 390 | + | |||
Deletion mutants | ||||||
pSVCC3 dE24 | IE72 | 132-274 | − | |||
pSVCC3 dS12 | IE72 | 345-end | + | |||
pSVCC3 dPst240 | IE72 | 1-38 | + |
aa, amino acid; wt, wild type