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. 1997 Jul 14;138(1):105–117. doi: 10.1083/jcb.138.1.105

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(a) Diagram of the Kiehart-Ellis chamber preparation, microneedle shape and alignment, and the mechanical severing motion. (b) Video image of the field of view. Shown is the glass microneedle tip pressing down on the coverslip surface and axoneme-nucleated microtubules. Bar, 5 μm.