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. 1997 Nov 17;139(4):865–873. doi: 10.1083/jcb.139.4.865

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2′5′A synthetase activity in peritubular cell and Sertoli cell extracts. 2′5′A synthetase enzymatic activity was determined in the different peritubular cell (a, P) and Sertoli cell (b, S) extracts as described in Materials and Methods. Cells were stimulated or not (C) with IFN α (50, 275, and 500 U/ml) or IFN γ (10, 100, 500, and 1,000 U/ml) for 14 h, or with Sendai virus (V100 and V500 U/ml) for 28 h. Each activity value is the mean of at least three totally independent experiments. Means between stimulated and control cells were compared by Student's t test: NS, P > 0.05; *, P < 0.02; **, P = 0.001; ***, P < 0.001.

2′5′A synthetase activity in peritubular cell and Sertoli cell extracts. 2′5′A synthetase enzymatic activity was determined in the different peritubular cell (a, P) and Sertoli cell (b, S) extracts as described in Materials and Methods. Cells were stimulated or not (C) with IFN α (50, 275, and 500 U/ml) or IFN γ (10, 100, 500, and 1,000 U/ml) for 14 h, or with Sendai virus (V100 and V500 U/ml) for 28 h. Each activity value is the mean of at least three totally independent experiments. Means between stimulated and control cells were compared by Student's t test: NS, P > 0.05; *, P < 0.02; **, P = 0.001; ***, P < 0.001.