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. 1997 Nov 17;139(4):865–873. doi: 10.1083/jcb.139.4.865

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Immunolocalization of PKR in peritubular and Sertoli cells. Cells were fixed after culture and permeabilized as described in Materials and Methods. Immunolocalization of PKR was performed using a rabbit polyclonal antibody against murine PKR and revealed using an avidin–biotin peroxydase complex amplification combination. Strong cytoplasmic staining was observed in both control peritubular cells (A) and Sertoli cells (C). Negative control for peritubular (B) and Sertoli cells (D) were performed using rabbit IgG at the dilution used for the PKR antibody. Bar, 15 μm.