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. 1997 Nov 17;139(4):865–873. doi: 10.1083/jcb.139.4.865

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Expression of Mx proteins in peritubular and Sertoli cells. Mx proteins were detected by immunoprecipitation. Cells were stimulated or not (C) with IFN α (50, 275, and 500 U/ml) or IFN γ (10 and 100 U/ml) for 14 h, or with Sendai virus (V100 and V500 U/ml) for 28 h. ³⁵S-labeled extracts were reacted with rabbit polyclonal antibody against rat/mouse Mx proteins, and products were analyzed on 7.5% polyacrylamide gel (a, peritubular cells, P; b, Sertoli cells, S). The positive and negative controls are represented by 3T3 cells transfected with the rat Mx1cDNA (3T3Mx1) and by 3T3 cells transfected with a plasmid lacking the MX1cDNA (3T3Neo), respectively. Blots shown are representative of three totally independent culture and immunoprecipitation experiments.