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. 1997 Nov 17;139(4):865–873. doi: 10.1083/jcb.139.4.865

Figure 8.

Figure 8

Immunolocalization of Mx proteins in peritubular and in Sertoli cells. Cells were fixed after culture and permeabilized as described in Materials and Methods. Immunolocalization of Mx proteins was performed using a mouse monoclonal antibody (2C12) against rat/mouse Mx proteins and revealed using an avidin–biotin peroxidase complex amplification combination. No staining was observed in either control peritubular cells (B) or Sertoli cells (D). In contrast, peritubular cells stimulated with 500 U/ml of Sendai virus (A) showed strong granular staining in the nucleus (arrows) and a more diffuse staining in the cytoplasm, whereas Sertoli cells stimulated with the same concentration of virus (C) showed a granular staining in the nucleus (arrows) and a diffuse and granular staining in the cytoplasm (arrowheads). Bar, 15 μm.