Table I.
Effects of n-Propyl Gallate, Syto 12, and FITC-Channel Excitation Light on Cultured Meiocytes*
| Treatment | NPG‡ | Syto 12§ | Light‖ | Viability (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Exp. 1 (6 h) | Exp. 2 (9 h) | |||||||||
| t = 0 | − | − | − | 71.2 | 73.1 | |||||
| A | − | − | − | 49.4 | 45.0 | |||||
| B | − | − | + | 38.9 | 27.5 | |||||
| C | − | + | − | 51.0 | 39.6 | |||||
| D | − | + | + | 32.6 | 32.5 | |||||
| F | + | + | + | 45.1 | 45.8 | |||||
*
Two different experiments are reported. Viability was measured immediately after extruding W23 meiocytes from anthers (t = 0) and after 6 h in the first experiment (Exp. 1) and after 9 h in the second experiment (Exp. 2). The average number of cells counted to determine percent viability was 213.
‡
0.25 mM n-propyl gallate.
§
2 μm Syto12.
‖
5-min exposure to 450- to 490-nm light at t = 0.