Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Nov 17;139(4):1033–1046. doi: 10.1083/jcb.139.4.1033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Levels of endogenous Xenopus α-catenin bound to cadherins decrease in embryos expressing the dominant-negative mutants αNcatNtermGFP or GFPαNcatNterm. Embryos were lysed in NP-40 buffer and immunoprecipitated using the monoclonal antibody to C-cadherin 6B6. The immunoprecipitates were immunoblotted with the polyclonal anti–αN-catenin antibody (CME). The lanes correspond to embryos injected with mRNA for 1, uninjected; 2, GFP; 3, αNcatNtermGFP; 4, GFPαNcatNterm; 5, uninjected. Lane 5 represents uninjected embryos subjected to immunoprecipitation with an anti-myc epitope antibody (9E10) as a negative control. Embryos were injected into both cells at the two-cell stage to obtain protein expression in the highest possible number of cells. Notice that the levels of endogenous α-catenin decrease significantly in lanes 3 and 4 compared to lanes 1 and 2.