Table III.

Full-Length α-Catenin Diminishes the Dorsalization caused by Xwnt-8 and the Axis Duplication Caused by β-Catenin

				DAI
mRNA Injected		# of Embryos		>8		7		6		5		Average DAI
0.125 ng Xwnt8 + 0.85 ng GFP		89		89		0		0		0		>8
0.125 ng Xwnt8 + 3 ng GFP		74		74		0		0		0		>8
0.125 ng Xwnt8 + 0.85 GFPαNcat		86		3		67		13		3		6.8
0.125 ng Xwnt8 + 3 ng GFPαNcat		98		5		36		55		0		6.3
0.125 ng GFP + 3 ng GFPαNcat		77		0		0		0		77		5
				No. with duplicated axes								Percent duplicated axes
0.02 ng β-catenin + 3 ng GFPαNcatCterm		142		67								47
0.02 ng β-catenin + 3 ng GFPαNcat		115		13								11

Numbers shown are sums taken from four separate experiments. Embryos were injected in one ventral blastomere at the four-cell stage. The degree of dorsalization (dorsoanterior index [DAI]) and axis duplication were scored at early tadpole stages. A score of 5 is given to a normal embryo; 10 means the embryo is completely dorsalized (as described by Kao and Elinson, 1988). In addition, when duplicate axes were seen in embryos injected with β-catenin + GFPαNcat, the axes were very much reduced, with no complete head structures when compared to the axes duplications seen in embryos injected with β + GFPαNcatCterm.