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Nuclear migration of β-subunit is saturable. Digitonin-permeabilized cells were incubated with 10 μl of testing solution containing 3 pmol GFP–β-subunit (a–d) or GFP-D450-876 (e–h) in the absence (a and e) or presence of either 24 pmol GST– β-subunit (b and f), GST-D450-876 (c and g), or GST-D3-447 (d and h) for 15 min on ice. Localization of GFP-chimeras was examined after fixation of the permeabilized cells as in Fig. 3 D.
