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. 1997 Nov 17;139(4):985–994. doi: 10.1083/jcb.139.4.985

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Microtubules exhibit dynamic instability. High resolution fluorescence images of unbudded G1 cells. Haploid strain 9dΔd lacks an endogenous copy of dynein and contains the GST–dynein- GFP fusion (pKBY701). A series of eight time points at 1-min intervals are displayed from top to bottom on the left and continuing on the right. Each image displayed represents a reconstruction from five exposures taken at 1-μm axial steps. A single microtubule at the bottom of each panel is shown to polymerize in the first three panels before undergoing a catastrophe and shortening in panel four. The same microtubule rescues and grows to the cell cortex before undergoing catastrophe and shortening back toward the spindle pole. Bar, 2 μm.