Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Nov 17;139(4):985–994. doi: 10.1083/jcb.139.4.985

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Nuclear movement requires cytoplasmic microtubules. Haploid strain 9dΔd (containing pKBY701) cells were treated with nocodazole (NZ) as described in the Materials and Methods. Five fluorescence images (from left to right) at 5-min intervals are shown. The cell on the lower left is small budded, and the poles have separated (note the asymmetry in intensity at the poles). The cell on the upper right has only one fluorescent SPB. No nuclear movements were detected throughout the analysis. Thus, nuclear motility is dependent upon intact cytoplasmic microtubules. Points of fluorescence, indicative of dynein-GFP, could be seen at the SPBs even upon prolonged NZ treatment. Short tufts of microtubules may be resistant to NZ treatment. Bar, 5 μm.