GUS activity observation in transgenic V. hirsuta roots and nodules. The activity of the GUS gene directed by the MsPG3-promoter region was visualized in complete young nodules and nodule primordia (A) and longitudinal cuts of mature nodules (B and C). Thick arrow, young nodule; thin arrows, nodule primordium; asterisks, meristematic zone; arrow heads, infection zone; SZ, symbiotic zone.