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. 2007 Dec 17;179(6):1095–1103. doi: 10.1083/jcb.200710058

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — The association between U2 genes and CBs was disrupted by the expression of a β-actin mutant. TetU2-45 cells were transfected with mCherry-lac repressor, reverse Tet-activator, and wild-type (wt) or dominant-negative (dn) YFP-actin constructs. (A) In the presence of wt YFP-actin, association of CBs and U2 arrays was not disrupted (arrow). However, in the presence of the nonpolymerizable dn YFP-actin, the association between CBs and the U2 array was significantly diminished. (B) Quantification of the relative repositioning of the U2 array within the chromosome 7 territory. Cells were scored (n > 85 cells per time point) on 3D z-axis projections as in Fig. 4 B in the presence of wt actin or dn actin. (C) Quantification of the effect of wt and dn YFP-actin on association of the U2 array with CBs. n > 250 cells. (D) The effect of dn YFP-actin on transcription of the U2 array as analyzed by quantitative PCR. The levels of 18S ribosomal RNA were monitored as a control. Bar, 2 μm.