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. 2007 Dec 17;179(6):1123–1131. doi: 10.1083/jcb.200703166

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Expression of an overactivated form of PKD fragments the Golgi apparatus. (A) HeLa cells stably expressing MannII-GFP were transfected with GST-tagged PKD WT, PKD-CAAX-WT, PKD-CAAX-CA, or PKD-CAAX-KD. The localization of PKD and the organization of Golgi membranes were monitored by fluorescence microscopy with anti-GST and TGN46 antibody, respectively. (B) PKD-CAAX-CA–dependent Golgi fragmentation requires DAG. HeLa MannII-GFP cells were pretreated with fumonisin1 (FB1) 24 h before transfection with PKD constructs. 24 h after transfection, cells were fixed and stained with anti-GST and anti-TGN46 antibodies.