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. 2007 Dec 17;179(6):1289–1300. doi: 10.1083/jcb.200705154

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Extracellular domain is sufficient for the synaptic localization of Fas2-YFP. (A) Schematic diagram of Fas2-YFP constructs. (B–D) Distribution of intact Fas2-YFP (B), extra–Fas2-YFP (C), and intra–Fas2-YFP (D) expressed on muscle 12 in wild-type embryos. Fas2-YFP strongly accumulated at the nascent postsynaptic sites of muscle 12 (arrows). (E) Quantification of the synaptic accumulation of different Fas2-YFP constructs in wild-type embryos at 15 h. ***, P < 0.001 by t test. Error bars represent SEM. (F–H) Distribution of intact Fas2-YFP (F), extra–Fas2-YFP (G), and intra–Fas2-YFP (H) expressed on all muscles in wild-type larvae. Strong (arrows) and weak (arrowheads) accumulation of Fas2-YFP constructs at the synaptic sites of muscle 12 are shown. Bars, 5 μm.