Skip to main content
. 2007 Dec 18;4(12):e337. doi: 10.1371/journal.pmed.0040337

Figure 4. Inhibition of P. vivax Invasion of Human Red Blood Cells by Anti-PvDBPII Ab.

Figure 4

Tests were performed to examine the influence of rabbit (A) and human (B) anti-PvDBPII Ab on P. vivax invasion on a patient sample cultured in PvCM plus 20% AB serum (Experiment 1) or a sample from a second patient cultured in PvCM plus 25% AB serum (Experiment 2). Control cultures (white bars) contained media without antibodies and were the same for both experiments. The concentration of antibodies (pre-bleed rabbit serum or nonspecific human IgG) in the positive control cultures (white bars) was equal to the highest concentration of test serum. Various concentrations of the test antibody (black bars) were added to late-stage P. vivax cultures and grown for 24 h (in duplicate), and the number of newly invaded cells was observed by light microscopy based on examination of 200 high-powered fields of Giemsa-stained thin smears or approximately 20,000 erythrocytes. Bars indicate mean ± standard deviation of the number of invasion events. p-Values (two-sided t-test) are shown for differences of p < 0.1 between test samples and their respective control antibody.