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. 2007 Nov 15;26(24):5048–5060. doi: 10.1038/sj.emboj.7601919

Figure 8.

Figure 8

Analysis of rDNA replication intermediates in 26T RNA base-pairing mutants. (A) Schematic of the palindromic rDNA 5′ NTS fragment generated by HinDIII digestion, and possible RI patterns following 2D gel electrophoresis. Simple Y arc: passive replication of 5′ NTS origins; bubble or bubble-to-Y arcs: initiation within the 5′ NTS; composite (simple Y arc and bubbles): active and passive replication of the 5′ NTS. (B) Southern blot analysis of HinDIII-digested DNA resolved on a neutral–neutral 2D gel. Thick right-pointing arrows point to bubble arcs. Thin left-pointing arrows point to simple Y arcs. (C) 2D gel analysis following enrichment for RIs on BND cellulose. (D) Southern blot analysis of total genomic DNA from 26T RNA transformants.