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. 2007 Nov 15;26(24):5033–5047. doi: 10.1038/sj.emboj.7601927

Figure 5.

Figure 5

Chl4RCENP−N or CENP-H depletion rescues the bipolar spindle defect in Mcm21RCENP−O-depleted cells. (A) Successive frames every 3 min from live-cell movies of H2B-GFP/α-tubulin-mRFP-expressing HeLa cells transfected with control, siMcm21R, siCENP-H, siChl4R, siMcm21R+Chl4R, siMcm21R+siCENP-H or partial siCENP-H (50% siRNA)+siMcm21R RNA. The composite images for H2B-GFP (green) and α-tubulin-mRFP (red) are shown. Scale bar=10 μm. (B) Quantification of bipolar spindle formation errors at T=12 min after NBD in cells treated with siRNA as indicated. (C) Protein levels of Mcm21R, CENP-H and Chl4R in cells treated with siRNA as indicated as measured by quantitative immunofluorescence. (D) Protein levels of Mcm21R and CENP-H in cells treated with control or partial siCENP-H (50% siRNA)+siMcm21R RNAs.