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. 2007 Nov 15;26(24):5033–5047. doi: 10.1038/sj.emboj.7601927

Figure 6.

Figure 6

The CENP-A NAC/CAD controls the levels of kinetochore-bound NDC80 complex. (A–C) Protein levels of Ndc80, CENP-E and Aurora B in cells treated with siRNAs as indicated by quantitative immunofluorescence as described in Figure 2. (D) Quantification of bipolar spindle formation errors at T=12 min after NBD in cells treated with siMcm21R RNA and DMSO or 2 μM ZM1. (E) Successive frames every 3 min from live-cell movies of H2B-GFP/α-tubulin-mRFP-expressing HeLa cells transfected with siMcm21R RNA and 2 μM ZM1. The composite images for H2B-GFP (green) and α-tubulin-mRFP (red) are shown. Scale bar=10 μm.