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. 2007 Nov 15;26(24):5033–5047. doi: 10.1038/sj.emboj.7601927

Figure 7.

Figure 7

Classification of further CENP-A NAC/CAD components. (A) Quantification of bipolar spindle formation errors at T=12 min after NBD in cells treated with control, siCENP-H, siCENP-I, siSim4R, siFta1R or siFta1R+siCENP-H RNAs. For comparison, values for control and siCENP-H-treated cells were transferred from Figure 5B. (B) Successive frames every 3 min from live-cell movies of H2B-GFP/α-tubulin-mRFP-expressing HeLa cells transfected with siFta1R or siFta1R+siCENP-H RNAs. The composite images for H2B-GFP (green) and α-tubulin-mRFP (red) are shown. Scale bar=10 μm. (C–E) Protein levels of Sim4R (C), CENP-I (D) and CENP-H (E) on kinetochores following treatment with control, siMcm21R, siCENP-H, siCENP-I or siSim4R RNA by quantitative immunofluorescence. (F) Protein levels of Mcm21R on kinetochores following treatment with control, siSim4R, siCENP-I, siSim4R+siMcm21R or siCENP-I+siMcm21R RNA by quantitative immunofluorescence. (G) Quantification of bipolar spindle formation errors at T=12 min after NBD in cells treated with control, siMcm21R, siMcm21R+siCENP-I or siMcm21R+siSim4R RNA. For comparison, values for control and siMcm21R-treated cells were transferred from Figure 5B (H) Protein levels of Mcm21R on kinetochores following treatment with control, siMcm21R or siFta1R RNA by quantitative immunofluorescence. (I, J) Protein levels of CENP-H (I) or Ndc80 (J) on kinetochores following treatment with control, siFta1R or siFta1R+siCENP-H RNA. (K) Percentage mitotic arrest in control, siMcm21R- or siFta1R-transfected cells with (+) or without (−) 16 h nocodazole treatment. (L) Successive frames every 3 min from live-cell movies of H2B-GFP/α-tubulin-mRFP-expressing HeLa cells transfected with siMcm21R+siSim4R or siMcm21R+siCENP-I RNA. The composite images for H2B-GFP (green) and α-tubulin-mRFP (red) are shown. Scale bar=10 μm.