Figure 2.

The Rh1 downregulation in tes-mutant flies is due to excessive endocytosis of Rh1. (A) The Rh1 endocytosis in tes flies depends on the shibire dynamin. During the 3-h light stimulation, both tes and the shi;tes double mutants were kept at 30.5°C, a temperature that prevents the mutant Shibire dynamin from function. (B) The shi mutation prevented light-induced Rh1 reduction in tes flies. The scaffold protein INAD was probed in parallel. For light stimulation in this particular experiment, the flies were exposed to pure blue light (750 lux) for three hours at 30.5°C. B, blue light. ^*Indicates that the sample is significantly different from others in the same group.