Fig. 1.

X-gal histochemistry of AxCALacZ-infected BMSC. a AxCALacZ was infected to BMSC at multiplicity of infection (MOI) 100. Twenty-four hours after infection, cells were incubated with X-gal reagent at 37°C for 3 h; 80% of AxCALacZ-infected BMSC was positive for X-gal staining, indicating high infection efficacy. b Western blot analysis of BDNF produced by adenovirus AxCABDNF. Bands of BDNF were detected in conditioned medium taken from plates with AxCABDNF-infected BMSC, but not in medium from normal and AxCALacZ-infected BMSC. Recombinant human BDNF (rhBDNF) was used as a positive control. Bar 30 μm