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. 1998 Feb 9;140(3):647–657. doi: 10.1083/jcb.140.3.647

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Production of mAb 297S that distinguishes T564-phosphorylated from nonphosphorylated radixin. Nonphosphorylated C-rad (100 ng; C-rad), T564-phosphorylated C-rad (100 ng; P-C-rad), and whole-cell lysate of Swiss 3T3 cells under conventional culture conditions (25 μg; Swiss 3T3 lysate) were immunoblotted with pAb TK89 (pAb TK89) or mAb 297S (mAb 297S). The former antibody recognized radixin as well as ezrin and moesin irrespective of their phosphorylation state, whereas the latter distinguished T564-phosphorylated C-rad from the nonphosphorylated molecule. The mAb 297S recognized ezrin and moesin that were phosphorylated at the corresponding threonine residues, T567 and T558, respectively.