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Immunogold electron microscopy of GAP-43–GFP-transporting vesicles. The axons were incubated with antiflag antibody followed by 5-nm colloidal gold conjugated second antibody. pl and m in a indicate plasma membrane and mitochondria, respectively. Arrows indicate some of the tubulovesicular organelles. Plasma membrane and tubular or vesicular organelles were labeled with gold particles. (c) Noninfected DRG neuron as a control. Only a few gold particles were seen. Bars, 0.2 μm.
